Campaspe Highway Patrol Sergeant Paul Nicoll said both vehicles were travelling south on the Northern Hwy when the accident happened.
"The truck went to turn left onto Middleton Rd before the sedan hit the truck," he said.
"At this stage we are investigating the cause of the collision."
A 76-year-old male, the driver of the car, and his 71-year-old female passenger were taken to Echuca hospital with minor injuries.
The 26-year-old male truck driver from Moama was also taken to Echuca hospital.
There are still minor delays for road users.
There has been an accident on the Northern Highway, between Echuca and Rochester.
A sedan collided with a small truck and ran off the road about 1.30pm.
Police and emergency services are currently helping to get the driver out of the car.
There are no road closures but lengthy delays.
More to come.