Train travellers will now be able to tap on and off using a Mastercard, Visa credit or debit card, as well as with their digital cards on smartphones or smartwatches.
The trial is only available to passengers travelling on a full fare, and will use specific readers and gates, which will be marked with which ones to use.
Tap and go will also be tested on the Craigieburn, Upfield and Ballarat lines.
“This public trial will ensure we have full confidence in the technology before it’s rolled out across the entire network,” Public and Active Transport Minister Gabrielle Williams said.
“Tap and go technology will make it quicker and easier for passengers to travel.”
The technology will only be activated on the trial lines, including stations in the City Loop.
Passengers planning to transfer to another train line, tram or bus must use a myki from the start of their journey to ensure they are not overcharged.
The trial is optional and will be rolled out throughout March and April, with passengers travelling on concession to have the opportunity to participate at later stages.
Once the trials are successfully completed, tap and go payments will be switched on across the rest of the rail network that uses myki, and will later be available on buses and trams.