Players plan to pause for 15 seconds after kick-off in matches in the ninth round held from Friday through Monday.
The Spanish soccer players' union said on Friday that "players will protest in a symbolic fashion to denounce the lack of transparency, dialogue and coherence of La Liga regarding the possibility of playing a game in the United States."
La Liga made official last week its plans to hold a the Barcelona-Villarreal game in Miami on December 20.
The union said the captains of the 20 top-flight sides were in agreement with the protest.Â
But the union added it had not asked the Barcelona and Villarreal players to participate in the protest, even though, the union said, "they share the basic premise of the protest."
Earlier on Friday, Barcelona coach Hansi Flick and his players made it clear they were not pleased at having to travel 7200km to play a regular-season game.
"My players are not happy, I am not happy, but La Liga decided that we will play this game," Flick said at a press conference.
Barcelona president Joan Laporta has defended the move by saying it represents an opportunity to further push into the American sports market.
But for Flick and his players, it represents added travel before a short winter break. Barcelona will also travel to Saudi Arabia for the Spanish Super Cup starting on January 7.
Barcelona midfielder Frenkie de Jong also criticised playing the game in the US, saying players were already overloaded with travel and a packed playing calendar.
La Liga president Javier Tebas has defended the game abroad as a key to boosting "revenues in the mid- to long-term" and increasing the value of his competition's television rights, which lag behind those of England's Premier League.
Tebas said the league plans to make an international match an annual event.
The match will be at Hard Rock Stadium in Miami Gardens, home of the NFL's Miami Dolphins.
The league has chartered planes to take to Florida what it estimates will be 2000 to 3000 fans from Villarreal, the nominal home team.
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