Victorian authorities are working to contact the families whose children attended the centres during Joshua Dale Brown's employment between August 2024 and February 2025.
The newly listed locations are all operated by Affinity Education: Kids Academy Waratah Estate in Mickleham, and Milestones Early Learning centres in Tarneit, Greensborough and Braybrook.
Brown was charged with more than 70 sex offences against eight children younger than two at a centre at Point Cook in Melbourne's southwest, between April 2022 and January 2023.
Police believe Brown worked at 23 childcare centres between January 2017 and May 2025, stretching from Williamstown to Sunbury.
Chief Health Officer Christian McGrath confirmed 830 additional families would be contacted, adding to the 1200 who have been urged to have precautionary testing.
"This is obviously a highly distressing situation and I want to reassure all families being contacted that the potential exposure risk to an infectious disease for their child remains low," Dr McGrath said on Tuesday.
Melody Glaister's daughter, now six, attended Creative Garden Early Learning Point Cook during Brown's employment at the centre.
She had been anxiously awaiting the results of her daughter's test, which came back negative on Tuesday.
"We're incredibly grateful and relieved as parents that our child is physically healthy but we are always going to wonder, did something happen to our child," Ms Glaister told AAP.
Ms Glaister said she could not believe more families must go through the traumatic process of testing.
Police also released an amended list of dates and locations, including for Papilio Early Learning in Essendon where the reported period of Brown's employment was extended by six months.
Authorities no longer believe the accused worked at Papilio's site in Hoppers Crossing between August 22, 2024 and March 12, 2025.
Families impacted by the date changes will be contacted by the health department from Tuesday.
Establishing a complete work history for Brown has been a key focus of the police investigation.
"This has been extremely complex due to childcare approved providers not having centralised records, requiring detectives to execute search warrants to obtain handwritten records, shift rosters and other critical information," Victoria Police said.
Since the charges came to light on July 1, police have received more than 270 Crime Stoppers reports.
An Affinity Education Group spokeswoman said the company had reviewed records to provide information to police about Brown's work history.
"We are deeply sorry for the distress this is causing our families, no family should have to go through this," they said.
It has been revealed Brown was the subject of two separate internal investigations in April 2023 and January 2024 at G8 Education, both unrelated to sexual abuse and occurring after the alleged incidents he was charged over.
The investigations took place before his employment with Affinity Education Group, which stated it was unaware of the investigations.
The two events were substantiated and reported to state authorities but Brown's working with children certificate remained in place.
If the substantiated allegations had been flagged and his working with children check was revoked in 2024, Ms Glaister believes fewer children would have needed to be tested.
"If the appropriate action had been taken, it's very likely there would be hundreds of parents right now that wouldn't be in the same situation," she said.
A Victorian government spokeswoman said health and mental health support was being offered to families.
1800 RESPECT (1800 737 732)
National Sexual Abuse and Redress Support Service 1800 211 028
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