Zelenskiy dismissed top aide Serhiy Shefir from his post of first assistant, where he had served since 2019.
The Ukrainian president also let go three advisers and two presidential representatives overseeing volunteer activities and soldiers' rights.
No explanation was given immediately for the latest changes in a wide-reaching personnel shake-up over recent months.
It included the dismissal on Tuesday of Oleksiy Danilov, who served as secretary of the National Security and Defence Council, and Valerii Zaluzhnyi as head of the armed forces on February 8.
Zaluzhnyi was appointed Ukraine's ambassador to the United Kingdom earlier this month.
Ukraine's air force said on Saturday that Russia launched 12 Shahed drones overnight, nine of which were shot down, and fired four missiles into eastern Ukraine.
Ukrainian officials say Russia has escalated its attacks on energy infrastructure in recent days. (AP PHOTO)
Russia unleashed a barrage of 38 missiles, 75 air strikes and 98 attacks from multiple rocket launchers over the last 24 hours, Ukraine's armed forces said in social media posts.
Two people were killed and one wounded in Russian shelling in Ukraine's partially occupied Donetsk province, regional governor Vadym Filashkin said on Saturday.
Ukrainian energy company Centrenergo said on Saturday that the Zmiiv Thermal Power Plant, one of the largest thermal power plants in the eastern Kharkiv region, was completely destroyed following Russian shelling last week.
Power outage schedules were still in place for about 120,000 people in the region, where 700,000 people had lost electricity after the plant was hit on March 22.
Russia has escalated its attacks on Ukrainian energy infrastructure in recent days, causing significant damage in several regions.
Officials in the Poltava region said on Saturday there had been "several hits" to an infrastructure facility, without specifying whether it was an energy facility.
13°C