The 40-year-old pop star had been due to play her third show at the Bell Centre in Montreal on Monday as part of her Mayhem Ball Tour but she called off the concert after being "strongly advised" not to perform by her doctor.
In a post on Instagram, she said: "Hi everyone, I'm so sorry to share that I'm unable to perform tonight and have to cancel the show. I've been fighting a respiratory infection for the past few days and doing everything I can to rest and recover, but it's gotten worse.
"My doctor has strongly advised me not to perform today and to be honest I don't think I could give you the quality of a performance today that you deserve.
"I know how deeply disappointing this is, and I truly could not feel worse about letting you down. I'm so sorry to everyone who made plans to be there and support me.
"Being in Montreal and performing for you on Thursday and Friday was magical and deeply meaningful. To everyone who was coming tonight, I'm absolutely heartbroken and so sorry."
Lady Gaga kicked off the tour in the US in July 2025 and the singer previously explained she had not planned to hit the road again so soon.
In a post on Instagram, she wrote: "I wasn't planning to tour this year after my shows in Singapore but the incredible response to the new album inspired me to keep things going."
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