Awaiting the Timbercutters in round one on April 4 is last season's runners-up Waaia and there is no doubting the Bombers will be out for blood.
But Mathoura coach Darcy Robinson said his side would be embracing the challenge.
“The game is going to show us where we are at straight away,” he said.
“We want to be playing against the best sides in the competition and this is a great opportunity for us straight away.
“In 2019, I thought we often prepared and played better against the stronger sides so this season will be about trying to find consistency against every team.”
Mathoura finished eighth last season and bowed out in the first week of finals.
The 2020 fixture will pit the Timbercutters against reigning premiers Strathmerton on two occasions - first in round five and then round 18.
While it may look a big test on paper, Robinson is backing his players to spring a surprise.
“There's no doubt Strathmerton will play deep into the finals again,” he said.
“We got close in 2019 and I think that experience will hold us in good stead in those types of games again.”
Mathoura will also face Deniliquin Rovers, Tocumwal and Yarroweyah on two occasions.
The round 13 trip to Rennie will be a chance for the Timbercutters to avenge last season's elimination final defeat.
The grand final will be held on September 12.
Mathoura fixture
Round 1: Mathoura v Waaia.
Round 2: Tocumwal v Mathoura.
Round 3: Mathoura v Yarroweyah.
Round 4: Mathoura v Deniliquin Rovers.
Round 5: Strathmerton v Mathoura.
Round 6: Mathoura v Picola.
Round 7: Katamatite v Mathoura.
Round 8: Bye.
Round 9: Mathoura v Jerilderie.
Round 10: Katunga v Mathoura.
Round 11: Mathoura v Blighty.
Round 12: Berrigan v Mathoura.
Round 13: Rennie v Mathoura.
Round 14: Bye.
Round 15: Mathoura v Tocumwal.
Round 16: Yarroweyah v Mathoura.
Round 17: Deniliquin Rovers v Mathoura.
Round 18: Mathoura v Strathmerton.
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