A report issued by Spain's Health Ministry before the MV Hondius docked in Tenerife confirmed the ship had cleared the appropriate health checks before laying anchor.
"According to the information provided by the experts who boarded the ship, the hygiene and environmental conditions are appropriate and they have not detected rodents so transmission by exposure to rodents on board is not likely," the report read.
Spanish passengers will leave the ship first, while countries including Belgium, France, Germany, Ireland, the United States, the UK and the Netherlands confirmed they had sent planes to evacuate their citizens aboard.
Passengers will not leave the boat until their allocated evacuation plane has arrived, Spanish officials said.
Passengers from the Netherlands will be the next group to leave the vessel, and their plane will also transport passengers from Germany, Belgium and Greece.
After that, passengers from Turkey, France, the UK and US. will be evacuated.
"The final flight of the operation is departing from Australia... It is the most complex flight and is scheduled to arrive tomorrow afternoon," Spanish Health Minister Monica Garcia said on Sunday.
That final flight will pick up six people from Australia, New Zealand and other Asian countries.
Thirty crew members will remain on board and sail to the Netherlands where the ship will be disinfected.
All passengers on the luxury cruise ship are considered high-risk contacts as a precautionary measure, Europe's public health agency said late on Saturday as part of its rapid scientific advice.
The ship left for Spain on Wednesday from the coast of Cape Verde after the World Health Organisation and European Union asked the country to manage the evacuation of passengers after the hantavirus outbreak was detected.
WHO Director-General Tedros Adhanom Ghebreyesus arrived on Saturday evening in Tenerife, alongside Spain's interior and health ministers and its minister for territorial policy, to co-ordinate the arrival of the ship.
The WHO said on Friday that eight people had fallen ill, including three who died - a Dutch couple and a German national.
Six of these people are confirmed to have contracted the virus, with another two suspected cases, the WHO said.
Hantavirus is usually spread by rodents but can in rare cases be transmitted person-to-person. The WHO has said the risk to the wider global population is low, but the risk to passengers and crew on the ship is moderate.
The ship was carrying 147 passengers and crew when a cluster of severe respiratory illnesses among passengers was reported to the WHO on May 3.
By then, 34 other passengers had departed the vessel, including one of four Australians, according to media reports, which first sailed from Argentina in March with stops in the Antarctic and other locations before heading north to waters off Cape Verde west of Africa.
The vessel was briefly held there after news of the outbreak emerged.
Four patients remained in hospital on Friday in South Africa, the Netherlands and Switzerland.
Oceanwide, the cruise operator, said there were no people with symptoms of a possible infection remaining on the vessel.
with Reuters