Trump criticised the presenter in a post on his social media platform Truth Social, calling for US media network ABC to "take a stand" against Kimmel.
It comes after a gunman attempted to storm the White House correspondents' dinner on Saturday night. Days before the incident, Kimmel did a sketch about the dinner on ABC's Jimmy Kimmel Live! where he joked that Melania had a "glow like an expectant widow".
Trump wrote on Truth Social: "Wow, Jimmy Kimmel, who is in no way funny as attested to by his terrible Television Ratings, made a statement on his Show that is really shocking.
"A day later a lunatic tried entering the ballroom of the White House Correspondents Dinner, loaded up with a shotgun, handgun, and many knives.
"He was there for a very obvious and sinister reason. I appreciate that so many people are incensed by Kimmel's despicable call to violence, and normally would not be responsive to anything that he said but, this is something far beyond the pale.
"Jimmy Kimmel should be immediately fired by Disney and ABC."
During the sketch, which aired last week prior to the dinner, Kimmel joked: "Our first lady, Melania, is here. So beautiful. Mrs Trump, you have a glow like an expectant widow."
Melania responded to the sketch in a post shared on X and described the talk show host as a "coward".
"Kimmel's hateful and violent rhetoric is intended to divide our country.
"His monologue about my family isn't comedy - his words are corrosive and deepen the political sickness within America.
"People like Kimmel shouldn't have the opportunity to enter our homes each evening to spread hate.
"A coward, Kimmel hides behind ABC because he knows the network will keep running cover to protect him.
"Enough is enough. It is time for ABC to take a stand. How many times will ABC's leadership enable Kimmel's atrocious behaviour at the expense of our community."
In September, the late-night presenter was taken off the air and suspended indefinitely following comments he made on the show after the murder of right-wing activist Charlie Kirk, an ally of Trump.
The suspension was met with criticism from the public, some of Hollywood's biggest stars and political figures, towards the US administration for infringing on their freedom of speech and free press, leading to Kimmel's return five days later.
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